Isolation and identification of Enterococcus faecium from Meat products with special reference to some virulence and antibiotics resistance factors.

Document Type : Research article

Authors

microbiology department , faculty of veterinary medicine, south valley university

Abstract

Enterococcus faecium is one of the most superbug pathogen which cause many diseases in human which include wound infection and endocarditis. also, this microorganism show high resistant to common antibiotics which used in hospital especially Vancomycin so the purpose of this study is detection E.Faecium from meat products which can be achieved by isolation and identification of E.faecium and detection some virulence gene and antibiotic resistance gene by PCR. Biochemical and molecular studies were performed on seventeen unidentified gram-stain positive, catalase and oxidase negative, Enterococcus-like organisms recovered from hundred meat products samples. The result of Vitek-2 compact system identified the suspected isolates as E. faecium. Antibiotic susceptibility testing of the isolates indicated resistance to most antibiotics, including penicillin, ampicillin, amoxicillin and vancomycin. The molecular analysis indicated the presence of virulence genes such as hyl (30%), ace (30%) and cylA (90%), and antimicrobial resistance genes as followed vanA gene (50%) and blaZ gene (90%). In conclusion meat products considered a good source of infection by vancomycin resistant Enterococcus Faecium (VRE) which have potential risk on human being so hygienic measurement are required during meat processing.

Keywords

Main Subjects

Files

Share

How to cite

Statistics